Coding

Part:BBa_K615004:Design

Designed by: Naomi Genuth   Group: iGEM11_Harvard   (2011-09-26)

Zif268 binding site-His3-URA3 construct for one-hybrid selection


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 15
    Illegal BglII site found at 528
    Illegal BglII site found at 588
    Illegal BamHI site found at 818
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1508
    Illegal SapI.rc site found at 1355


Design Notes

Zif268 binding site in the promoter region to initiate expression of His3 and URA3 only when a zinc finger is bound properly.

Note: two illegal Pst1 sites (bases 2-7 and 812-817) were removed using Quikchange, but those sites have not yet been sequence verified.


Source

The sequence was synthesized.

References